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Objective To investigate the distribution and dynamic changes of both Wnt signaling molecules and CK15 throughout the three phases of the follicular cycle,and to explore the relationship between Wnt/β-catenin signaling and CK15 in rat whisker hair follicle(HF) growth cycles.Methods Hematoxylin-Eosin(HE) and immunofluorescence stains were used to characterize the expression patterns,including sites and levels of some representative proteins of both canonical and non-canonical Wnt signaling molecules,as well as HF epithelial stem cell marker CK15.Results The expression patterns of both β-catenin and Wnt5a were correlated with that of CK15.CK15 was only expressed in anagen.In catagen,β-catenin showe damassive depletion while Wnt5a noticeably increased.In telogen,high level expression of β-catenin and low level of Wnt5a were detected.Wnt10b and TCF3 were detected during the entire HF growth cycle.Conclusion The seresults suggest that Wnt5a is associated with the transition of anagen-catagen phase,accompanied by broad deletion of β-catenin and loss of CK15.Wnt10b is important for the main tenance of HF activity and is related to the telogen-anagen transition.