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应用体细胞核移植方法制备ApoCⅢ转基因小型猪,并分析其表型特征。通过血浆脂质检测、口服脂肪负荷试验、肝素后血浆脂蛋白酶活性测定以及静脉葡萄糖耐量试验,分析转基因小型猪的脂质和糖代谢特点。该小型猪表现出显著的高甘油三酯血症,与非转基因小型猪相比,转基因小型猪对甘油三酯(TG)的清除显著延迟,且肝素后血浆脂蛋白脂酶活性也显著降低,但静脉糖耐量未有改变。
The somatic cell nuclear transfer method was used to prepare ApoC Ⅲ transgenic minipig and its phenotypic characteristics were analyzed. Lipid and glucose metabolism in transgenic miniature pigs were analyzed by plasma lipid test, oral fat load test, heparin plasma lipoproteinase activity test and venous glucose tolerance test. The miniature pig showed marked hypertriglyceridemia, the TG was significantly delayed in transgenic miniature pigs compared with non-transgenic miniature pigs, and the plasma lipoprotein lipase activity was also significantly decreased after heparinization, However, intravenous glucose tolerance has not changed.