论文部分内容阅读
作者在低温条件下(4-8℃)用蔗聚糖-泛影葡胺分离液分离SLE患者外周血单核细胞,然后置37℃水浴经不同时间处理,观察温度变化使单核细胞上Fc受体及C3受体脱落的情况。用经亚凝集效价的兔抗SRBC血清中IgG包被SRBC(sSRBC)与单核细胞作EA玫瑰花试验,以检测单核细
The authors isolated peripheral blood mononuclear cells from patients with SLE at low temperature (4-8 ° C) using Sysmex-diatrizoate meglumine and then treated with water at 37 ° C for different periods of time. Changes in temperature were observed to allow monocytes to gain Fc Receptors and C3 receptors off the situation. IgG-coated SRBC (sSRBC) and monocytes were subcultured with EA rosette in sub-aggregated titer rabbit anti-SRBC serum to detect mononuclear