论文部分内容阅读
取外科手术切除的新鲜胃腺癌组织标本22例,分离单个胃癌细胞,反复冻融后经0.22μm微孔滤膜过滤,获冻融液作为粗提可溶性抗原.用该抗原刺激患者自身外周血单个核细胞,与抗CD3单克隆抗体和重组白细胞介素2一起共同培养,体外诱导细胞毒T淋巴细胞(CTL),并用流式细胞仪测定其培养前后的表型变化.分别用自身胃癌细胞、人胃腺癌细胞株(BGC-823)和人胃粘液腺癌细胞株(MGC-803)作为靶细胞,用MTT比色法测定CTL的体外杀伤活性.结果表明,此种方法诱导的CTL对自体癌细胞杀伤活性为89.53%±3.1%,明显高于对胃癌细胞株的杀伤活性(P<0.01);对同一组织分型的胃腺癌细胞株BGC-823的杀伤活性为63.05%±5.6%,高于对MGC-803的杀伤活性40.09%±
Twenty-two fresh gastric adenocarcinoma tissue specimens were surgically resected and individual gastric cancer cells were isolated. After repeated freezing and thawing, they were filtered through a 0.22 μm microporous membrane to obtain freeze-thaw fluid as the crude soluble antigen. The patient’s own peripheral blood was stimulated with the antigen. The nucleus cells were co-cultured with anti-CD3 monoclonal antibody and recombinant interleukin 2 to induce cytotoxic T lymphocytes (CTL) in vitro, and the phenotypic changes before and after culture were measured by flow cytometry. Human gastric adenocarcinoma cell line (BGC-823) and human gastric mucinous adenocarcinoma cell line (MGC-803) were used as target cells. MTT assay was used to determine the killing activity of CTL in vitro. The results showed that this method induced CTL to autologous The killing activity of cancer cells was 89.53%±3.1%, which was significantly higher than that of gastric cancer cell lines (P<0.01). The killing activity of gastric cancer cell line BGC-823 with the same tissue typing was 63.05%±5.6%. Higher than the killing activity of MGC-803 40.09%