目的　克隆鼠转化生长因子 β1(TGF β1)基因并研究其在转染的成骨细胞中表达情况。方法　采用逆转录 聚合酶链式反应 (RT PCR)可从大鼠淋巴细胞中扩增出 1.2Kb特异性片段 ,经酶切鉴定证实为鼠TGF β1cDNA。将此片段插入 5 .4Kb的 pcDNA表达载体 ,构建得到 6 .6Kb的pcDNA TGF β1重组质粒。应用脂质体介导的基因转移技术将TGF β1表达载体导入鼠成骨细胞 ,48h后用SABC法进行瞬时表达的检测。结果　 48h后转染的成骨细胞具有明显鼠TGF β1基因的表达产物。结论　将构建的TGF β1表达载体转入鼠成骨细胞 ,48h后可获得TGF β1的高效表达。 Objective To clone the transforming growth factor β1 (TGFβ1) gene and study its expression in transfected osteoblasts. Methods The specific fragment of 1.2Kb was amplified from rat lymphocytes by reverse transcriptase-polymerase chain reaction (RT-PCR) and confirmed to be rat TGFβ1 cDNA by restriction enzyme digestion. The fragment was inserted into a 5 .4Kb pcDNA expression vector to construct a 6. 6Kb pcDNA TGF β1 recombinant plasmid. The TGFβ1 expression vector was transfected into mouse osteoblasts using liposome-mediated gene transfer technique, and transient expression was detected by SABC method 48h later. Results After 48 h, the transfected osteoblasts had the obvious expression product of rat TGF β1 gene. Conclusion The constructed TGF β1 expression vector was transfected into rat osteoblasts, and high expression of TGF β1 was obtained after 48 h.