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目的:研究左旋千金藤立定(SPD)调节6-羟基多巴胺(6-OHDA)损毁大鼠纹状体的Fos、前脑腓肽(PENK)和前强啡肽(PDYN)mRNA表达水平与旋转行为的关系.方法:用免疫细胞化学法观察Fos表达,用地高辛非同位素法标记的寡核苷酸探针检测纹状体PENK和PDYN mRNA,进行图像处理作半定量分析.结果:(1)SPD给予1,3,7 d后,损毁大鼠旋转行为仍维持在高水平;(2)SPD诱导双侧纹状体Fos显著表达,尤以损侧为甚.SPD重复应用使双侧纹状体的Fos诱导表达下降,尤以健侧为显著;(3)与健侧相比,损毁侧纹状体的PENK mRNA表达水平增加非常显著.SPD重复应用7 d,使这种增加的PENK mRNA水平明显下降.同时,SPD也使健侧PENK mRNA水平降低.然而,6-OHDA损毁和SPD处理对双侧纹状体的PDYN mRNA水平无明显影响.结论:SPD激发6-OHDA损毁大鼠旋转行为维持在高水平,与损侧纹状体的Fos表达和PENK mRNA水平下降是同步的.但是,6-OHDA损毁和SPD均未显示出对PDYN mRNA表达的影响.
OBJECTIVE: To investigate the expression of Fos, PENK and PDYN mRNA in the striatum of 6-hydroxydopamine (6-OHDA) -deficient rats and the rotational behavior .Methods: The expression of Fos was observed by immunocytochemistry, and the DNA and DNA of PENK and PDYN were detected by digoxigenin-labeled oligonucleotide probe.All the images were processed for semi-quantitative analysis.Results: (1) SPD induced a significant increase of Fos expression in bilateral striatum, especially in the lesion side after SPD administration for 1,3,7 d (3) Compared with the contralateral side, the increased expression of PENK mRNA in the injured lateral striatum was significantly increased.During 7 days, the increased expression of PENK mRNA However, the level of PENK mRNA was decreased in SPD group.However, 6-OHDA damage and SPD treatment had no significant effect on PDYN mRNA level in bilateral striatum.Conclusion: SPD-induced 6-OHDA-lesioned rat spin Behaviorally maintained at a high level, in synchronicity with decreased Fos expression and reduced PENK mRNA levels in the striatum. However, both 6-OHDA damage and SPD The effect on PDYN mRNA expression was not shown.