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目的建立太子参药材中氨基酸类成分的柱前衍生-高效液相色谱指纹图谱分析方法,为整体控制和评价太子参的质量提供依据。方法采用柱前衍生-高效液相色谱法分别对11批太子参进行检测,色谱柱为Hypersil ODS色谱柱(5μm,4.6 mm×20 cm);流动相:醋酸钠溶液-乙腈-甲醇系统,梯度洗脱;体积流量为1.0 ml·min-1;检测波长为338 nm;柱温:25℃;进样体积:1μl;采用药典委员会指定的指纹图谱相似度评价软件(2004 A版)进行相似度评价,计算相似度。结果建立了太子参的指纹图谱,共有12个共有峰,并达到较好分离。该分析方法具有很好的精密度、重现性和稳定性,均符合指纹图谱的技术要求。11批太子参的相似度为0.915~0.999。结论方法稳定可靠,重复性好,可为控制和评价太子参的质量提供依据。
Objective To establish a method of pre-column derivatization-high performance liquid chromatography (HPLC) fingerprinting of amino acids in Radix et Rhizoma Rhei and provide the basis for the overall control and evaluation of the quality of Radix Pseudostellariae Radix. Methods 11 samples of Pseudostellaria heterophylla were detected by precolumn derivatization - high performance liquid chromatography. The chromatographic column was Hypersil ODS column (5μm, 4.6 mm × 20 cm). The mobile phase consisted of sodium acetate solution - acetonitrile - methanol system. The gradient The volume of flow was 1.0 ml · min-1, the detection wavelength was 338 nm, the column temperature was 25 ℃, and the injection volume was 1μl. The similarity of the fingerprints similarity evaluation software (2004 A) designated by Pharmacopoeia Commission Evaluation, calculation of similarity. Results The fingerprint of Radix Pseudostellaria heterophylla was established, with a total of 12 common peaks and a better separation. The analysis method has good precision, reproducibility and stability, are in line with the technical requirements of the fingerprint. The similarity of 11 batches of heterophylla was 0.915 ~ 0.999. Conclusion The method is stable and reliable with good repeatability, which can provide the basis for controlling and evaluating the quality of Radix Pseudostellariae Radix.