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采用酶催化法,以7-氨基-3-[(Z)-烯丙-1-基]-3-头孢环-4-羧酸(7-APCA)为母核,D-对羟苯甘氨酸甲酯(D-HPGME)为酰基供体,水作溶剂,在固定化青霉素G酰化酶的催化作用下合成头孢丙烯。本研究中催化酶的载体采用氨基和环氧载体,筛选合适的酶活,考察反应pH、温度、投酶量、投料比、底物浓度等多个因素对酶法合成头孢丙烯的影响。通过对酶法合成头孢丙烯的工艺条件的优化,为头孢丙烯绿色生产技术的推行打下基础。
The enzyme-catalyzed method was used to synthesize D-p-hydroxyphenylglycine methyl ester with 7-amino-3 - [(Z) -allyl- Ester (D-HPGME) is an acyl donor and water is used as a solvent to synthesize cefprozil under the catalysis of immobilized penicillin G acylase. In this study, the catalytic enzyme carrier using amino and epoxy carrier, screening the appropriate enzyme activity, investigated the reaction pH, temperature, the amount of enzyme, feed ratio, substrate concentration and other factors on the synthesis of cefprozil by the enzyme. Optimization of the process conditions for the synthesis of cefprozil enzymatically laid the foundation for the implementation of cefprozil green production technology.