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目的研究人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染后NK细胞及各亚群分泌IFN-γ,表达CD107a和释放颗粒酶B的功能变化。方法选取未经高效抗逆转录病毒疗法(highly active antiretroviral therapy,HAART)治疗的HIV慢性感染者,按照CD4+T细胞数量分成高、低2组,用流式细胞仪检测其外周血NK细胞及各亚群IFN-γ、CD107a和颗粒酶B的表达情况。结果 CD4+T细胞高组NK细胞及各亚群分泌IFN-γ和CD107a的水平较HIV抗体阴性对照组高(P=0.034,P=0.045),颗粒酶B释放百分比高于HIV抗体阴性对照组和CD4+T细胞低组(P=0.004,P=0.028),其IFN-γ+NK细胞百分比与病毒载量呈显著负相关(r=-0.382,P=0.015)。结论 NK细胞功能增强可能是控制HIV感染疾病进程的因素之一。
Objective To study the functional changes of NK cells and their subsets secreting IFN-γ, expressing CD107a and releasing granzyme B after infection with human immunodeficiency virus (HIV). Methods Chronic HIV positive patients without HAART were divided into two groups according to the number of CD4 + T cells. Flow cytometry was used to detect the levels of NK cells in peripheral blood and The subpopulations of IFN-γ, CD107a and granzyme B expression. Results The levels of IFN-γ and CD107a secreted by CD4 + T cells in high NK cells and their subpopulations were higher than those in HIV negative controls (P = 0.034, P = 0.045). The percent release of Granzyme B was higher than that in HIV negative control (P = 0.004, P = 0.028). The percentage of IFN-γ + NK cells was negatively correlated with the viral load (r = -0.382, P = 0.015). Conclusion NK cell function may be one of the factors that control the process of HIV infection.