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目的:研究使用不同时间和剂量的雷公藤多甙(GTW)对昆明小鼠怀孕率的影响及肉苁蓉干预和停用GTW后的自愈倾向。方法:将90只成年雄性昆明鼠随机分为9组(每组10只)。对照组给予1%羧甲基纤维素(CMC)溶液;GTW各组分别给予45.0、30.0、22.5、15.0、7.5mg/(kg·d)GTW混悬液;肉苁蓉各组分别给予30.0、7.5mg/(kg·d)GTW混悬液;停药组给予30.0mg/(kg·d)GTW混悬液。以上各组先灌胃20d,20d后与成年雌鼠1∶2合笼7d。第28d起GTW各组按原剂量继续给药;肉苁蓉各组给原剂量GTW混悬液的同时分别给10.0、2.5g/(kg·d)肉苁蓉煎液;停药组停GTW,改为1%CMC溶液。20d后再次合笼7d。每次合笼后观察雌鼠怀孕率。结果:随着GTW给药时间的延长和剂量的增加,雌鼠怀孕率呈下降趋势。GTW45.0mg/(kg·d)组给药40d后怀孕率降为0%(P<0.01)。肉苁蓉10.0g/(kg·d)组怀孕率由20d时的25%上升到40d时的55%。停药组怀孕率由25%上升到56%,与对照组比较,差异无显著性(P>0.05)。结论:GTW对雄性小鼠的生殖毒性呈时间和剂量依赖性。肉苁蓉可以改善GTW对雄鼠生殖系统的抑制作用,提高雌鼠的怀孕率。停用GTW20d,怀孕率可以基本恢复正常。
OBJECTIVE: To study the effects of GTW on the pregnancy rate of Kunming mice and the tendency of Cistanche tuberculosis after intervention and GTW withdrawal. Methods: Ninety adult male Kunming mice were randomly divided into 9 groups (10 in each group). The control group was given 1% carboxymethylcellulose (CMC) solution. The GTW suspensions of 45.0, 30.0, 22.5, 15.0 and 7.5 mg / (kg · d) / (kg · d) GTW suspension; the withdrawal group was given 30.0mg / (kg · d) GTW suspension. The above groups were given gavage for 20 days, and after 20 days, adult female rats were kept 1: 2 for 7 days. The GTW groups were administered with the same dosage as the original dose on the 28th day; the Cistanche tuberculosis groups were given GTX at 10.0 and 2.5g / (kg · d) % CMC solution. After 20d again cage 7d. After each cage observed the pregnancy rate of female rats. Results: With the prolongation of GTW administration and dose increase, the pregnancy rate of female rats showed a decreasing trend. The pregnancy rate was reduced to 0% (P <0.01) 40 days after GTW 45.0 mg / (kg · d) administration. Cistanche 10.0g / (kg · d) group pregnancy rate increased from 25% at 20d to 55% at 40d. The rate of pregnancy in the withdrawal group increased from 25% to 56%, with no significant difference compared with the control group (P> 0.05). Conclusion: The reproductive toxicity of GTW to male mice is time-and dose-dependent. Cistanche can improve the GTW male reproductive system inhibition, improve the pregnancy rate of females. GTW20d disabled, the pregnancy rate can return to normal.