OBJECTIVE:To investigate the efficacy of Fig fruit powder and olive on hepatic,renal and splenic inju-ry induced by 2-nitropropane (2-NP) in mice,espe-cially if they were used in combination.METHODS:A total of 40 adult BALB/c male mice weighting 25-30 g/each.Mice were categorized in-to five groups (8 each).Group 1 as negative con-trol.Group 2 as positive control group intraperito-neally injected with 2-NP (100 mg/kg b.w.) 3 times/weekly for eight weeks.Group 3 injected with 2-NP and were orally supplemented with Fig(300 mg/kg).Group 4 injected with 2-NP and were orally supplemented with olive (100 mg/kg).Group 5 injected with 2-NP and were orally sup-plemented with mixture of Fig and olive (3∶1 re-spectively).RESULTS:Histopathological observation of liver in mice treated with 2-NP showed cellular degenera-tion,pyknosis,and congestion of the portal vein.In kidney there were disorganization of the cortical tissues,cellular necrosis and plenty of inflammato-ry lymphocytic aggregation.Significant elevations in liver function parameters (alanine aminotransfer-ase and aspartate aminotransferase),mRNA expres-sion levels of tumor necrosis factor-α,nicotinamide adenine dinucleotide phosphate oxidase and cyclo-oxygenase were detected as anti-inflammatory markers and 5-lipoxygenase,interleukin-1β and in-terleukin-6 as inflammatory biomarkers for liver and spleen,also significant elevations was detect-ed in lipid peroxidation levels.The levels of antioxi-dants,glutathione,glutathione peroxidase,cata-lase and superoxide dismutase were significantly decreased.CONCLUSION:our findings indicated that Fig fruit powder and olive protected against hepatic,renal and splenic injury induced with 2-NP in mice,espe-cially if they were used in combination.