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[目的]研究TCP在苜蓿种植土壤中的降解作用,为氯酚类物质污染土壤生物修复技术的实际应用提供依据。[方法]利用玻璃房盆栽试验,研究苜蓿对土壤中2,4,6-三氯酚(TCP)污染的修复作用以及苜蓿的生长情况和TCP对土壤多酚氧化酶、脱氢酶和过氧化氢酶活性的影响。[结果]苜蓿经过75d的生长后,在低、中、高3个浓度处理中,土壤中TCP含量均在15d内迅速降低,随后降低速度逐渐变缓;在苜蓿生长30d时,3个处理的苜蓿鲜重与对照间无显著差异(P<0.05),而在生长75d时,各处理的苜蓿鲜重明显对照低(P<0.05),表明土壤中TCP对苜蓿的生长具有抑制作用;苜蓿能显著提高土壤中多酚氧化酶、脱氢酶和过氧化氢酶的活性,从而提高了土壤植物和微生物对污染物的降解能力。[结论]苜蓿能促进土壤酶活性的提高,促进土壤中有机物的降解,从而可以利用苜蓿进行TCP污染土壤的植物修复。
[Objective] The research aimed to study the degradation of TCP in the alfalfa planting soil and provide the basis for the practical application of bioremediation technology of chlorophenols contaminated soil. [Method] The pot experiment of glass house was used to study the effect of alfalfa on the remediation of 2,4,6-trichlorophenol (TCP) in soil and the growth of alfalfa and the effect of TCP on soil polyphenol oxidase, dehydrogenase and peroxidation Effect of Hydrogenase Activity. [Result] The results showed that the TCP content of alfalfa decreased rapidly within 15 days after low, medium and high concentrations of 3 treatments. After the alfalfa was grown for 30 days, The fresh weight of alfalfa was significantly lower than that of the control (P <0.05), while the fresh weight of alfalfa was significantly lower than that of the control (P <0.05) at the 75th day of growth, indicating that TCP inhibited the growth of alfalfa in soil. Significantly increase the activity of polyphenol oxidase, dehydrogenase and catalase in soil, thereby improving the degradation ability of soil plants and microorganisms to pollutants. [Conclusion] Medicago sativa could promote the increase of soil enzyme activity and promote the degradation of organic matter in soil, so alfalfa could be phytoremediation of soil contaminated by TCP.