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应用本院免疫研究室制备的抗前S_2单克隆抗体、ABC法,检测各型乙型肝炎78例肝组织标本前S_2的表达情况,并与其它乙肝病毒抗原的显现进行了比较。78例肝标本中,前S_2阳性者45例,阳性率为57.69％。前S_2的定位及表达形式与HBsAg相似,即:前S_2定位于肝细胞和肝癌癌细胞胞浆以及胆管上皮细胞;前S_2在肝细胞的表达形式可分为:弥漫型、包涵体型、膜下型及胞膜型。前S_2胞膜型表达与肝病的活动性相关连。HBcAg阳性组前S_2阳性率显著高于HBcAg阴性组,这提示肝内前S_2可能是HBV复制的标志。
Anti-pre-S 2 monoclonal antibody prepared by our hospital immunization laboratory was used to detect the expression of S_2 in 78 samples of various types of hepatitis B before and after comparison with other hepatitis B virus antigens. In 78 cases of liver specimens, there were 45 cases of positive former S 2, the positive rate was 57.69%. The localization and expression of pre-S_2 was similar to that of HBsAg, ie, the former S_2 was located in the cytoplasm of hepatocellular carcinoma and hepatocellular carcinoma and the bile duct epithelial cells. The expression pattern of pre-S 2 in hepatocytes could be divided into diffuse type, inclusion type, Type and membrane type. The former S 2 membrane type expression is associated with the activity of liver disease. The positive rate of pre-S 2 in HBcAg-positive group was significantly higher than that in HBcAg-negative group, suggesting that intrahepatic pre-S 2 may be a marker of HBV replication.