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[目的]研究3种外源氧化酶液和H2O2处理对水曲柳体胚发生的影响。[方法]利用不同浓度多酚氧化酶(polyphenol oxidase,PPO)、葡萄糖氧化酶(glucose oxidase,GOD)、超氧化物歧化酶(superoxide dismutase,SOD)溶液和过氧化氢(Hydrogen peroxide,H2O2)处理未成熟水曲柳合子胚子叶,探索不同处理对水曲柳体胚发生中子叶外植体生长、褐化以及体胚发生的影响,通过对比分析不同处理在水曲柳体胚发生中的作用,揭示水曲柳体胚发生中外植体褐化与体胚发生的关系。[结果]H2O2处理使水曲柳外植体褐化提前发生,且抑制了外植体的生长和体胚发生;不同浓度PPO处理促进外植体生长和褐化,并提高体胚发生率;GOD和SOD处理均提高外植体的褐化率;经过酶处理的外植体,当体胚发生提前时,各体胚发生率都很低,而当处理30d的体胚诱导率与处理60d的体胚诱导率相差较大时,其体胚发生率较高。[结论]该研究结果为提高水曲柳体胚诱导率、改善水曲柳体胚发生状况和优化水曲柳体胚发生系统奠定了基础。
[Objective] The research aimed to study the effects of three kinds of exogenous oxidase and H2O2 treatment on the somatic embryogenesis of Ash. [Method] With different concentrations of polyphenol oxidase (PPO), glucose oxidase (GOD), superoxide dismutase (SOD) and hydrogen peroxide (H2O2) The effects of different treatments on the growth, browning and somatic embryogenesis of somaclonal neocotyl explants were investigated. The effects of different treatments on somatic embryogenesis , Revealing the relationship between the explants browning and somatic embryogenesis in the somatic embryogenesis of Ash. [Result] H2O2 treatment prematurely induced browning of explants and inhibited the growth and somatic embryogenesis of explants. Different concentrations of PPO promoted the growth and browning of explants and increased the incidence of somatic embryos. The GOD and SOD treatment increased the browning rate of explants; the enzyme-treated explants, when the somatic embryogenesis in advance, the incidence of somatic embryos are very low, and when the 30d the induction of somatic embryos and treated 60d body When the induction rate of embryos is quite different, the incidence of somatic embryos is higher. [Conclusion] The results of this study laid the foundation for improving the induction rate of somatic embryos, improving the somatic embryogenesis status and optimizing the somatic embryogenesis system.