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目的 探讨特发性血小板减少性紫癜(ITP)患者血小板表面人类白细胞抗原 (HLA- DR)的表达和CD4+T淋巴细胞的活化状态。方法 采用流式细胞术检测 60例ITP患者和 60例正常人血小板表面HLA DR的表达水平和自身血小板活化的淋巴细胞产生白细胞介素 2(IL -2)水平。结果 ITP患者血小板表面HLA- DR的表达量为 0 .808%±0 .218%,正常对照组为 0. 025%±0. 019%,两者相比差异具有统计学意义(P<0. 01)。ITP患者CD4+T淋巴细胞经自身血小板激活后,IL 2的表达量为 5 .1% ±1 23%,较正常对照组(0 .0% )有明显差异(P<0 .01)。结论 ITP患者在自身血小板HLA DR抗原的作用下,出现了自身反应性T淋巴细胞的异常活化,T淋巴细胞处于高活性状态。
Objective To investigate the expression of human leukocyte antigen (HLA-DR) on platelets and the activation of CD4 + T lymphocytes in patients with idiopathic thrombocytopenic purpura (ITP). Methods Flow cytometry was used to detect the expression of HLA DR on platelets in 60 patients with ITP and 60 normal controls and the level of interleukin 2 (IL -2) produced by platelet-activated lymphocytes. Results The expression level of HLA-DR on platelet of ITP patients was 0.808% ± 0.188%, while the control group was 0.025% ± 0.19%. There was significant difference between the two groups (P <0.05). 01). The expression of IL-2 in CD4 + T lymphocytes of ITP patients after self-platelet activation was 5.1% ± 1.23%, which was significantly lower than that of the normal control group (0.0%) (P <0.01). Conclusion ITP patients under the action of their own platelet HLA DR antigen, there was abnormal activation of autoreactive T lymphocytes, T lymphocytes in a highly active state.