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[目的]探讨芍药软肝方对H22肝癌、S180肉瘤荷瘤小鼠的抑瘤作用,并初步探索其作用机制。[方法]采用H22肝癌、S180肉瘤移植瘤小鼠模型,分荷瘤阴性对照组(生理盐水)、芍药软肝方低、中、高剂量组和阳性对照组(环磷酰胺,CTX),观察芍药软肝方对两种荷瘤小鼠肿瘤生长的抑制作用以及芍药软肝方对H22肝癌荷瘤小鼠生存时间的影响;并运用荧光定量RT-PCR法评价芍药软肝方对H22肝癌荷瘤小鼠肿瘤组织cyclinD1表达的作用。[结果]芍药软肝方低、中、高剂量组和CTX组对移植性荷H22肝癌小鼠的抑瘤率分别为17.72%、33.99%、23.73%、43.95%;与生理盐水组相比,芍药软肝方低、中、高剂量组和CTX组瘤重均有显著性差异(P<0.01)。芍药软肝方低、中、高剂量组和CTX组对移植性荷S180肉瘤小鼠的抑制率分别为29.59%、31.34%、11.17%、53.92%;与生理盐水组相比,低剂量组和中剂量组瘤重均有显著性差异(P<0.05)。芍药软肝方各给药组对H22肝癌腹水瘤小鼠均有一定的生命延长作用,中剂量组与生理盐水组生存天数相比(18.42±2.50vs15.75±2.42),具有显著差异(P=0.014)。芍药软肝方能下调H22肝癌小鼠肿瘤组织cyclinD1 mRNA的表达水平,低剂量组或中剂量组与生理盐水组相比有显著差异(P=0.031,P=0.012),高剂量组与生理盐水组相比差异非常显著(P=0.004)。[结论]芍药软肝方对H22肝癌、S180肉瘤荷瘤小鼠有明显的抑瘤作用,其抑瘤作用可能与下调肿瘤组织cyclinD1mRNA表达水平有关。
[Objective] To investigate the antitumor effect of Shaoyaogangan decoction on H22 hepatoma and S180 sarcoma-bearing mice, and to explore its mechanism. [Methods] H22 hepatocellular carcinoma, S180 sarcoma xenograft model in mice was divided into two groups: low dose, middle dose and high dose of Shaoyuan Rougan Decoction (positive control group) and positive control group (CTX) Shaoyao Ruangan Decoction on tumor growth in two tumor-bearing mice and Paoyao Ruangan prescription on the survival time of H22 hepatoma tumor-bearing mice; and evaluate the effect of Shaoyao Ruangan Decoction on H22 liver cancer load The role of cyclinD1 expression in tumor-bearing mice. [Results] The inhibition rates of Shaoyao Ruangan Fang low, medium and high dose CTX group and transplanted H22 hepatoma mice were 17.72%, 33.99%, 23.73% and 43.95%, respectively. Compared with saline group, Paeonfunangan Fang low, medium and high dose group and CTX tumor weight were significant differences (P <0.01). The inhibition rates of Shaoyao Rougan Fang low, medium and high dose group and CTX group were 29.59%, 31.34%, 11.17% and 53.92% respectively in mice bearing S180 sarcoma. Compared with saline group, The tumor weight in the middle dose group was significantly different (P <0.05). Each treatment group of Shaoyaogangan decoction had certain life prolongation effect on H22 hepatoma ascites tumor mice. Compared with the survival time of saline group (18.42 ± 2.50vs15.75 ± 2.42), there was a significant difference (P = 0.014). Paoyuan Ruangan Decoction can down-regulate the expression of cyclinD1 mRNA in H22 hepatoma mice, there is a significant difference (P = 0.031, P = 0.012) between low dose group and middle dose group and normal saline group The difference between the groups was significant (P = 0.004). [Conclusion] Shaoyao Ruangan Decoction has obvious anti-tumor effect on H22 hepatoma and S180 sarcoma-bearing mice, and its anti-tumor effect may be related to the down-regulation of cyclinD1 mRNA expression.