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目的:观察心肌肥大形成过程中细胞周期蛋白D1(cyclinD1)基因表达的时相性变化,并探讨cyclinD1在心肌肥大形成中的作用。方法:采用腹主动脉缩窄法建立大鼠心肌肥大模型,设缩窄手术组及假手术对照组,观察两组大鼠手术后不同时间心脏左心室(含左心室游离壁与室间隔)质量与体质量比、左心室游离壁厚度及心肌细胞横径的变化,以确证模型的稳定建立;其后应用RNA印迹法分析检测cyclinD1基因表达的变化。结果:手术后2周时两组大鼠的心脏左心室质量与体质量比、左心室游离壁厚度以及心肌细胞横径均无显著差别,而缩窄手术组cyclinD1基因mRNA表达显著高于对照组(P<0.05);4周时缩窄组大鼠上述3项病理学检测指标及cyclinD1基因mRNA表达均高于对照组(P<0.05)。结论:在心肌肥大形成过程中cyclinD1基因mRNA表达增加,且早于病理变化出现,提示cyclinD1可能参与了心肌肥大的形成。
OBJECTIVE: To observe the changes of cyclinD1 gene expression in myocardial hypertrophy, and to explore the role of cyclinD1 in myocardial hypertrophy. Methods: The rat model of cardiac hypertrophy was established by abdominal aorta constriction. The rats in the narrowing and sham operation groups were divided into two groups. The left ventricular (including left ventricular free wall and interventricular septum) And body mass ratio, left ventricular free wall thickness and myocardial cell diameter changes in order to confirm the establishment of a stable model; then detected by Northern blot analysis of cyclinD1 gene expression changes. Results: At 2 weeks after operation, there was no significant difference in left ventricular mass to body mass ratio, left ventricular free wall thickness, and myocardial cell diameter between the two groups. The mRNA expression of cyclinD1 in the stenosis group was significantly higher than that in the control group (P <0.05). At the 4th week, the above three indexes of pathological examination and the expression of cyclinD1 mRNA were all higher than those of the control group (P <0.05). Conclusion: The expression of cyclinD1 mRNA increased during cardiac hypertrophy and earlier than pathological changes, suggesting that cyclinD1 may be involved in the formation of cardiac hypertrophy.