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目的:建立高效液相色谱法同时测定鸡矢藤中熊果酸和齐墩果酸的含量。方法:色谱柱为DiamonsilTM C18(250×4.6 mm,5滋m),流动相为甲醇-0.05mol.L-1磷酸溶液(93∶7)为流动相,检测波长210 nm,进样量10滋L。结果:熊果酸在21.28滋g.mL-1～212.8滋g.mL-1范围内线性关系良好(r=0.9997),平均回收率为97.79%(RSD=1.30%);齐墩果酸在24.02滋g.mL-1～240.2滋g.mL-1范围内线性关系良好(r=0.9996),平均回收率为98.36%(RSD=1.11%)。结论:该方法简便、灵敏、准确,可用于鸡矢藤药材的质量评价。
Objective: To establish a HPLC method for the simultaneous determination of ursolic acid and oleanolic acid in Vater. METHODS: The mobile phase consisted of DiamonsilTM C18 (250 × 4.6 mm, 5 μm) with a mobile phase of methanol-0.05 mol·L-1 phosphoric acid (93:7) at the detection wavelength of 210 nm. L. Results: Ursolic acid showed a good linear relationship (r = 0.9997) with an average recovery of 97.79% (RSD = 1.30%) in the range of 21.28μg · mL-1 ~ 212.8μg · mL- The linear relationship was good (r = 0.9996) with an average recovery of 98.36% (RSD = 1.11%) in the range of 24.02 g · mL -1 to 240.2 g · mL -1. Conclusion: The method is simple, sensitive and accurate and can be used for quality evaluation of Fructus Juniperus.