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目的:探讨血管生长因子受体3(vascular endothelial growth factor 3,VEGFR3)在ART妊娠胎盘组织中的差异表达及其安全性研究中的意义。方法:收集11例自然妊娠(对照组)和10例ART妊娠(ART组)的单胎足月妊娠选择性剖宫产分娩的胎盘组织,分析比较组间的临床资料;并应用免疫组织化学技术和实时荧光定量PCR技术,观察VEGFR3在胎盘组织的细胞定位,比较VEGFR3在ART和对照组胎盘组织中的差异表达。结果:VEGFR3在胎盘组织中定位于胎盘绒毛合体滋养层细胞胞质。与对照组相比,VEGFR3细胞定位一致,ART妊娠的胎盘组织中VEGFR3 mRNA表达水平有上升趋势,但无统计学差异(P>0.05)。结论:ART妊娠的胎盘组织中VEGFR3细胞定位和表达水平未见明显变化。
Objective: To investigate the differential expression of vascular endothelial growth factor 3 (VEGFR3) in placenta of ART during pregnancy and its significance in the safety study. Methods: The placenta tissue of 11 cases of spontaneous cesarean section delivered by cesarean section in pregnancy (control group) and 10 cases of ART pregnancy (ART group) were collected. The clinical data of the cases were analyzed and compared. Immunohistochemistry And real-time fluorescence quantitative PCR were used to observe the cellular localization of VEGFR3 in placental tissue. The differential expression of VEGFR3 in ART and control group was compared. Results: VEGFR3 localized in the placenta of the placental syncytiotrophoblast cytoplasm. Compared with the control group, the location of VEGFR3 cells was consistent, and the expression level of VEGFR3 mRNA in ART pregnancy placenta had an upward trend, but there was no significant difference (P> 0.05). Conclusion: The location and expression level of VEGFR3 in placenta of ART pregnancy did not change obviously.