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H1N1流感病毒的聚合酶具有RNA复制、转录等功能,并且对流感病毒片段包装、子代繁殖及宿主适应性等有着重要作用。通过分析人、猪及禽类H1N1流感病毒聚合酶片段的二核苷酸频率及同义密码子的偏爱性,发现不同宿主中,流感病毒聚合酶片段的CpG频率最低,且均被强烈抑制;通过三类宿主间的比较发现,人流感病毒抑制最为强烈,且其CpG频率随年份呈下降趋势,但2009年毒株的CpG频率突然上升。比较同义密码子使用频率发现,含有CpG的同义密码子相对使用频率均小于1,证明CpG抑制作用是影响同义密码子偏爱性的一个重要因素。以上结果暗示,CpG抑制对H1N1流感病毒的进化及跨宿主传播可能有重要影响。
H1N1 influenza virus polymerase RNA replication, transcription and other functions, and the influenza virus fragment packaging, offspring reproduction and host adaptability plays an important role. By analyzing the dinucleotide frequencies and the synonymous codon preferences of human, swine and poultry H1N1 influenza virus polymerase fragments, it was found that the CpG frequency of the influenza virus polymerase fragment was the lowest among all the hosts and was strongly inhibited; by Comparison of the three types of hosts found that human influenza virus was the most strongly inhibited, and its CpG frequency showed a downward trend year by year, but in 2009 the CpG frequency suddenly increased. Comparing the frequency of synonymous codons, the relative frequency of synonymous codons containing CpG was found to be less than 1, demonstrating that CpG inhibition is an important factor affecting the preference of synonymous codons. The above results suggest that CpG inhibition may have a significant impact on the evolution and cross-host transmission of the H1N1 influenza virus.