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目前关于嗜酸复红染色在诊断早期心肌损伤有一实际价值,但条件不易掌握,容易出现假阴假性和假阳性,与真阳性难以区别,不同程度地影响正确诊断。为此,我们在大白鼠人工心肌梗死实验采用本法的过程中,为提高其分辨力,作了一些探索,取得较为满意的效果,现将方法及体会介绍如下: 1 染液 A液:0.2%甲酚紫(甲酚固紫)10ml 1%草酸 O.2ml 蒸馏水 40ml 先分别配制好,单独保存。甲酚紫(cresy1 truly violet)须充分溶解,
At present, there is a practical value in the diagnosis of early myocardial injury with eosin staining, but the conditions are not easy to grasp. False negatives and false positives are easily appeared, which are difficult to distinguish from true positives and affect the correct diagnosis to varying degrees. For this reason, we made some explorations and obtained satisfactory results when using this method in experiments of artificial myocardial infarction in rats. The methods and experiences are introduced as follows: 1 dye solution A: 0.2 % Cresyl violet (cresyl violet) 10ml 1% oxalic acid O.2ml distilled water 40ml were first prepared, stored separately. Cresyl violet (cresy1 truly violet) to be fully dissolved,