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为观察白细胞介素（ＩＬ）－３、ＩＬ－６基因疗法对化疗后造血恢复及重建的作用，研究了成纤维细胞介导的ＩＬ－３、ＩＬ－６基因疗法以及合用两种基因疗法对５－氟尿嘧啶所造成的造血损伤小鼠模型的恢复作用。结果发现，单用ＩＬ－３基因疗法对化疗后小鼠中性粒细胞及白细胞减少具有促恢复作用，但对血小板促恢复作用不明显；单用ＩＬ－６基因疗法对化疗后小鼠血小板及中性粒细胞减少具有促恢复作用；当合用ＩＬ－３、ＩＬ－６基因疗法时可使化疗后小鼠血小板、中性粒细胞及白细胞提前恢复到化疗前水平，尤其血小板恢复作用更明显，且最低水平时血小板和中性粒细胞数量高于单一疗法时的数量，但合用对白细胞恢复作用效果与单用ＩＬ－３基因疗法相比无显著差异。对小鼠骨髓ＣＦＵ－ＧＭ及ＣＦＵ－ＭＫ进行体外动态观察，发现合用疗法促进ＣＦＵ－ＧＭ、ＣＦＵ－ＭＫ恢复作用显著高于单一疗法。结果表明合用ＩＬ－３、ＩＬ－６基因疗法能显著恢复化疗导致的造血损伤，促进化疗后血细胞恢复的疗效显著优于单用ＩＬ－３或ＩＬ－６基因疗法，可望为恢复肿瘤患者放、化疗导致的机体造血损伤提供新的途径。
In order to observe the effects of interleukin (IL)-3, IL-6 gene therapy on hematopoietic recovery and reconstruction after chemotherapy, fibroblast-mediated IL-3, IL-6 gene therapy and two gene therapy combinations were studied. Recovery of hematopoietic damage induced by 5-fluorouracil in a mouse model. The results showed that IL-3 gene therapy alone could promote the recovery of neutrophils and leukocytes in mice after chemotherapy, but it had no obvious effect on the recovery of platelets; IL-6 gene therapy alone could treat platelets in mice after chemotherapy. Neutrophil reduction has a pro-restorative effect; when combined with IL-3 and IL-6 gene therapy, mouse platelets, neutrophils, and leukocytes can be restored to pre-chemotherapy levels in advance after chemotherapy, especially platelet recovery. At the lowest level, the number of platelets and neutrophils was higher than that at the time of monotherapy, but the effect of combined use on leukocyte recovery was not significantly different from that of IL-3 gene therapy alone. The in vitro observation of mouse bone marrow CFU-GM and CFU-MK showed that combination therapy promoted the recovery of CFU-GM and CFU-MK significantly higher than that of monotherapy. The results showed that combination of IL-3 and IL-6 gene therapy can significantly restore the hematopoietic damage caused by chemotherapy, and the effect of promoting blood cell recovery after chemotherapy is significantly better than that of IL-3 or IL-6 gene therapy alone, which is expected to restore the tumor patients. , Chemotherapy induced hematopoietic damage in the body provides a new way.