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用含有转座子Tn5的铜绿假单胞杆菌PAO1826(pMO75::Tn5,Kmr)诱变我国马铃薯青枯菌小种3号菌株PO41,获得胞外多糖正常产生的接合子6000多个,经SDS-PAGE电泳筛选出胞外蛋白减少1～9种的菌株21株。经测试其中20株为原养型菌株,1株为营养缺陷型菌株。这21株突变株在烟草叶片上呈现典型的过敏性反应。用茎部毛细管滴注法在马铃薯植株上进行致病力测试的结果表明,胞外蛋白发生减少的21株突变株中有20株致病力均不同程度下降,通过分析突变株胞外蛋白缺失种类与致病力强弱的关系,初步确定59.5ku、55ku和47ku的胞外蛋白在青枯菌致病过程中起着十分重要的作用。
The Pseudomonas solanacearum strain PO41 was mutagenized with Pseudomonas aeruginosa PAO1826 (pMO75 :: Tn5, Kmr) containing transposon Tn5 to obtain more than 6,000 conjugates normally produced by extracellular polysaccharide. SDS -PAGE electrophoresis screened 21 strains of extracellular protein reduced by 1 to 9 strains. Twenty of the tested strains were prototrophic strains and one strain was auxotrophic. These 21 mutant strains showed a typical allergic reaction on tobacco leaves. The results of pathogenicity test on potato by stem-capillary instillation showed that 20 of 21 mutants with decreased extracellular proteins decreased in varying degrees. By analyzing the deletion of extracellular protein Species and virulence of the relationship between the initial determination of 59.5ku, 55ku and 47ku extracellular proteins play a very important role in the pathogenesis of R. solanacearum.