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目的:探索中国苏南地区汉族人群血清单核细胞趋化蛋白(MCP)-1水平与该蛋白基因-2518G/A多态性和急性心肌梗死(AMI)的相关性。方法:对94例AMI患者(AMI组)和73例经冠脉造影检查证实非冠心病者(对照组)采用PCR-RFLP法检测MCP-1-2518G/A多态性,采用ELISA法检测血清MCP-1水平。结果:血清MCP-1水平(M/IQR)在AMI组(186.24/285.15 pg/ml)显著高于对照组(100.71/134.02 pg/ml)(P=0.001)。AMI和非冠心病患者及其亚组男性和女性、老年和非老年患者的组内任何基因型之间,血清MCP-1水平(M/IQR)均无显著差异(P均>0.05)。多元线性回归分析揭示血清MCP-1水平与性别、吸烟、高血压史和糖尿病史等相关,与MCP-1-2518位点携带G等位基因无显著相关。校正年龄、性别、吸烟、高血压史、糖尿病史、血脂水平和血清MCP-1水平等因素后,高于等于75%分位数的血清MCP-1水平与AMI的发病风险呈正相关[OR(95%CI):2.764(1.061-7.204),P=0.038]。结论:在中国苏南地区汉族人群中AMI患者血清MCP-1水平显著升高;MCP-1水平与吸烟、高血压和糖尿病相关,与男性负相关,与MCP-1-2518G/A多态性无显著相关;高于等于75%分位数的MCP-1血清水平可能是AMI的一个独立危险因素。
Objective: To explore the correlation between serum monocyte chemoattractant protein (MCP-1) level and the gene -2518G / A polymorphism and acute myocardial infarction (AMI) in Chinese Han population of southern Jiangsu Province. Methods: The polymorphisms of MCP-1-2518G / A were detected by PCR-RFLP in 94 patients with AMI (AMI group) and 73 patients with coronary artery disease confirmed by coronary angiography (control group). The serum levels of MCP- MCP-1 level. Results: The serum MCP-1 level (M / IQR) was significantly higher in the AMI group (186.24 / 285.15 pg / ml) than that in the control group (100.71 / 134.02 pg / ml) There were no significant differences in serum MCP-1 levels (M / IQR) between AMI and non-CHD patients and their subgroups of male and female, elderly and non-elderly patients in any genotype. Multivariate linear regression analysis revealed that serum MCP-1 level correlated with sex, smoking, history of hypertension and history of diabetes, and had no significant correlation with MCP-1-2518 carrying G allele. After adjusting for age, sex, smoking, history of hypertension, history of diabetes, serum lipids and serum MCP-1, serum MCP-1 levels above the 75% quantile were positively correlated with the risk of AMI [OR 95% CI): 2.764 (1.061-7.204), P = 0.038]. Conclusion: The level of serum MCP-1 in AMI patients was significantly increased in Han population of southern Jiangsu province. The level of MCP-1 was correlated with smoking, hypertension and diabetes, negatively correlated with male and MCP-1-2518G / A polymorphism No significant correlation; MCP-1 serum levels above the 75% quantile may be an independent risk factor for AMI.