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目的：测定精子中3β-羟基甾体脱氢酶（HSD）。方法：采用酶组织化学方法对27例精子活率正常和20例精子活率低下的男子精子进行了研究。结果：3β-HSD主要分布在精子颈部，其着色面积不等，精子活率正常组3β-HSD着色面积为616．48±108．48μm2／100个精子，精子活率低下组为354．37±50．58μm2／100个精子，两组差异极显著（P＜0．001）。结论：3β-HSD可能通过某种途径间接参与精子的运动。
Objective: To determine 3β-hydroxy steroid dehydrogenase (HSD) in sperm. Methods: Enzymatic histochemistry was used to study the sperm motility of 27 sperm mothers and 20 sperm motility patients. Results: The 3β-HSD mainly distributed in the sperm neck, and its coloring area varied. The area of 3β-HSD coloring in normal sperm motility group was 616.48 ± 108.48μm2 / 100 sperm, sperm motility was low in 354.37 ± 50.58μm2 / 100 sperm, the difference between the two groups was significant (P <0.001). Conclusion: 3β-HSD may be indirectly involved in sperm motility by some means.