具有分化能力的烟草花粉植株叶片愈伤组织,经过13个月的继代培养,其分化能力随继代次数的增加而逐渐丧失。与此同时,单倍体细胞逐渐减少,产生大量多倍体和非整倍体细胞。发现不同激素水平的处理,对愈伤组织的分化和染色体数目变异的效应也不同。 Differentiation of tobacco leaf pollen callus, after 13 months of subculture, its differentiation ability gradually decreased with the increase of the number of subcultures. In the meantime, the number of haploid cells is gradually reduced, resulting in a large number of polyploidy and aneuploidy cells. It was found that the effects of different hormonal levels on callus differentiation and chromosome number variation were also different.