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Objective:To evaluatethemicrosatelliteinstability(MSI),expressionof mismatchrepair(MMR)gene(hMLH1,hMSH2)andproliferationkineticsincolorectalcancer(CRC)withfamilialpredisposition.Method:Forty-sixcasesof CRC were studiedusingsilverstainingpolymerasechainreaction-singlestrandconformation polymorphism(PCR-SSCP)technique,streptavidin-peroxidase(SP)immunohistochemicalmethodand flowcyto-metry.Results:In CRCpatientswithfamilialpredisposition,theMSI-positiveratewas higherthanin sporadicCRC(P<0.05).FamilialpredispositionandpositiveMSIwerestronglyrelatedto earlyageof canceronset,theproclivity for proximalcoloniccancer,poordifferentiatedandextracolorectalnmalignancies(P<0.01,P<0.05).Theincidence of negativeexpressionof hMLH1intumortissueandhMLH1,hMSH2innormalcolorectaltissueswassignificantly higher(P<0.05).Thenegativeexpressionof hMLH1togetherwithhMSH2was relatedwithpositiveMSI(P<0.05).InMSI-positiveCRCcells,theproliferationcellnucleusantigen(PCNA)expression,proliferationindexandS-phase cellsdecreasedobviously(P<0.01,P<0.05).Conclusion:In CRCwithfamilialpredisposition,MSI mightbe an importantcontributor.Therateof hMLH1andhMSH2mutationincreasedintumorandnormaltissue,andtheproli-ferationactivityof theircancercellwaslower.
Objective: To evaluate the microsatellite instability (MSI), expression of mismatch repair (MMR)gene (hMLH1, hMSH2) and proliferation kineticsin colorectal cancer (CRC) with familial predisposition. Method: Forty-six cases of CRC were processed usingsilverstaining polymerase chainreaction-singlestrandconformation polymorphism (PCR-SSCP)technique, streptavidin-peroxidase (SP) immunohistochemical method flowcyto-metry.Results: In CRCpatientswithfamilialpredisposition, theMSI-positiveratewas higherthanin sporadicCRC (P <0.05) .FamilialpredispositionandpositiveMSIwerestronglyrelatedto earlyageof canceronset, theproclivity for proximalcoloniccancer, poordifferentiatedandextracolorectalnmalignancies (P <0.01, P <0.05) .Theincidence of negativeexpressionof hMLH1intumortissueandhMLH1, hMSH2innormalcolorectaltissueswassignificantly higher (P <0.05 ).Thenegativeexpressionof hMLH1togetherwithhMSH2was relatedwithpositiveMSI(P<0.05).InMSI-positiveCRCcells,theproliferationcellnucleusantigen(PCNA)expression,proliferationindexandS-phase cellsdecre Asedobviously (P<0.01, P<0.05). Conclusion: In CRCwithfamilialpredisposition, MSI might be an important contributor. Therateof hMLH1andhMSH2mutationincreasedintumorandnormaltissue, andtheproli-ferationactivity of theircancercellwaslower.