目的测定白花银背藤根和茎中东莨菪内酯的含量。方法采用高效液相色谱法,以C18化学键合硅胶柱分离东莨菪内酯,甲醇-0.5%冰醋酸(40∶60)为流动相,检测波长为345nm进行测定。结果白花银背藤根和茎中的东莨菪内酯含量差异较大。结论根中的东莨菪内酯含量比茎中含量高。 Objective To determine the content of terpene lactone in the roots and stems of B. sinensis. Methods High performance liquid chromatography was used to separate scopoletin from C18 chemically bonded silica gel column. Methanol-0.5% glacial acetic acid (40∶60) was used as the mobile phase and the detection wavelength was 345 nm. Results The contents of scopoletin in the roots and stems of Bletilla platyphylla were quite different. Conclusion The content of scopoletin in roots is higher than that in stems.