目的　探讨猪与人皮肤组织蛋白抗原性的差异。方法　人和小猪全厚正常皮肤作冰冻切片 ,用抗人皮肤组织相关抗原 (HLA ABC ,HLA DR ,Vimentin ,Desmin ,Actin ,FⅧRA ,Cytokeratin)、细胞因子及其受体和胞外基质 (Hyalu ronicacid ,Fibronectin ,Laminin ,TypeⅣcollagen ,Estradiol 17β和Testosterone)及Ⅰ Ⅲ Ⅶ型胶原等抗原成分的单克隆或多克隆抗体进行免疫组化染色 ,比较猪与人皮肤组织抗原性异同。结果　在猪皮肤组织可检测到I Ⅲ Ⅳ Ⅶ型胶原和Hyaluronicacid、Fibronectin、Laminin、FⅧRA、Cytokeratin、IL 6、IL 8、Estradiol 17β、Testosterone及ICAM 1;多克隆抗体阳检率明显高于单克隆抗体 ;其中细胞外基质成分和分子量较小的细胞因子 ,以及结构或功能原始的蛋白分子在猪与人皮肤有较好的同源性 ,而某些蛋白分子 (如细胞因子或激素的受体和淋巴细胞CD分子等 )则多为阴性 ,显示抗体 抗原结合反应的特异性及其种族差异。结论　猪与人皮肤组织抗原之间存在很大的差异 ,猪血管内皮细胞表达人ICAM 1,提示二者粘附分子可能存在物种间的交叉 ,可能与异种皮肤移植难以存活有关。本研究为异种脱细胞真皮基质的临床应用提供了有用的资料 Objective To investigate the difference of protein antigenicity between pigs and human skin. Methods Frozen sections were obtained from normal skin of full thickness human and piglets. Anti-human skin antigens (HLA ABC, HLA DR, Vimentin, Desmin, Actin, FⅧRA and Cytokeratin), cytokines and their receptors and extracellular matrix ronicacid, Fibronectin, Laminin, TypeⅣcollagen, Estradiol 17β, and Testosterone) and type Ⅰ ¢ ócollagen were detected by immunohistochemistry to compare the antigenicity between pigs and human skin. Results The positive rate of polyclonal antibody was significantly higher than that of the monoclonal antibody in pig skin (P <0.05). The positive rate of polyclonal antibody was significantly higher than that of monoclonal antibody Antibodies; cytokines, including extracellular matrix components and small molecular weight, as well as structural or functional original protein molecules in pigs and human skin have better homology, and some protein molecules (such as cytokines or hormone receptors And lymphocyte CD molecules, etc.) are mostly negative, showing the specificity of the antibody antigen binding reaction and race differences. Conclusion There is a great difference between human and human skin antigens in pigs. The expression of human ICAM 1 in pig vascular endothelial cells suggests that there may exist inter-species crosses between the two adhesion molecules, which may be related to the difficult survival of xenograft skin graft. This study provides useful information for the clinical application of xenogenic acellular dermal matrix