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目的:探讨核仁素在大鼠心肌缺血预适应(Ischemic Preconditioning,IPC)中的表达情况及其作用。方法:采用在体大鼠结扎冠状动脉前降支5min后,再灌注10min,再结扎5min,再灌注10min,构建心肌缺血预适应的动物模型;采用低剂量过氧化氢(0.1mM)预处理原代培养的新生大鼠心肌细胞90min
Objective: To investigate the expression of nucleolin in rat ischemic preconditioning (Ischemic Preconditioning) and its role. Methods: Rat model of myocardial ischemic preconditioning was established by ligation of the anterior descending coronary artery in rats for 5 min, reperfusion for 10 min, ligation for 5 min and reperfusion for 10 min. Low-dose hydrogen peroxide (0.1 mM) Primary cultured neonatal rat cardiomyocytes 90min