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目的:研究双氢青蒿素在单壁碳纳米管修饰电极上的电化学行为及测定。方法:制备单壁碳纳米管(SWCNT)修饰玻碳电极,并用扫描电子显微(SEM)法、伏安法、电化学交流阻抗法对所制备的修饰电极进行表征。以此为工作电极,用线性扫描伏安法测定双氢青蒿素。结果:在pH=7.0,0.1mol·L-1的磷酸盐缓冲溶液(PBS)中,5.0×10-4mol·L-1双氢青蒿素在单壁碳纳米管修饰玻碳电极上还原峰电位为-0.85V,比同浓度双氢青蒿素在裸玻碳电极上的峰电位正移了270mV,峰电流显著增大。在优化实验条件下,还原峰电流与双氢青蒿素浓度在5.0×10-7～5.0×10-4mol·L-1的范围内呈线性关系,检出限为3.0×10-7mol·L-1。对5.0×10-4mol·L-1的双氢青蒿素平行测定10次的RSD为1.7%。结论:该方法简单、快速、灵敏,可用于含双氢青蒿素样品的含量测定。
OBJECTIVE: To study the electrochemical behavior and determination of dihydroartemisinin single-walled carbon nanotubes (CNTs) modified electrodes. Methods: Single-walled carbon nanotubes (SWCNTs) were prepared to modify glassy carbon electrodes. The modified electrodes were characterized by scanning electron microscopy (SEM), voltammetry and electrochemical impedance spectroscopy. Using this as a working electrode, dihydroartemisinin was determined by linear sweep voltammetry. Results: In the phosphate buffer solution (PBS) with pH = 7.0 and 0.1 mol·L-1, 5.0 × 10-4 mol·L-1 dihydroartemisinin reduced the peak at the single-walled carbon nanotubes modified glassy carbon electrode Potential of -0.85V, than the same concentration of dihydroartemisinin bare glassy carbon electrode positive shift 270mV peak current was significantly increased. Under the optimized experimental conditions, the reduction peak current was linear with the concentration of dihydroartemisinin in the range of 5.0 × 10-7 ~ 5.0 × 10-4mol·L-1 with the detection limit of 3.0 × 10-7mol·L -1. The RSD of 10 parallel determinations of 5.0 × 10 -4 mol·L -1 dihydroartemisinin was 1.7%. Conclusion: The method is simple, rapid and sensitive and can be used to determine the content of dihydroartemisinin samples.