利用鲎试剂中被激活的凝固醇水解人工合成的鲎三肽(Boc—leu—Gly—Ars—PNA),释出对硝基苯胺,经重氮化反应后与盐酸萘乙二胺生成红色的偶氮染料,45nm5在波长处测定光密度,适当条件下酶原激活量与生成的偶氮染料的吸收度呈线性关系,间接反应被测定内毒素浓度.实验中比较了十一个输液样品热原试验的免法,凝胶法和合成基质法的鲎试验,结果,基质法在灵敏度,准确度等方面具有更多优点。 Nitrobenzidine is released by hydrolyzing the synthetic tripeptide (Boc-leu-Gly-Ars-PNA) by the coagulant activated by limulus reagent and diazotized with naphthylethylenediamine hydrochloride to produce red The azo dye, 45nm5, was used to determine the optical density at the wavelength. Under appropriate conditions, the amount of zymogen activated was linear with the absorbance of the generated azo dye, and the indirect reaction was used to determine the endotoxin concentration. The original test-free, gel method and the synthetic matrix method 鲎 test, the results of matrix method has more advantages in terms of sensitivity, accuracy and so on.