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目的 探讨高胆固醇血症大鼠该模型中骨调素 (Osteopontin ,OPN)和单核细胞趋化因子 -1(Monocytechemoattractantprotein -1,MCP -1)的表达及其意义。方法 2 5只Wistar大鼠被随机分为 2组 :对照组 (C组 ,10只 ) ,高脂组 (H组 ,15只 )。C组饲以普通鼠料 ,H组饲以含 4%胆固醇和 1%胆酸钠的高脂鼠料 ,12周后检测血清各脂质成分、2 4h尿蛋白、内生肌酐清除率 ,HE及PAS染色观察肾脏病理改变 ,免疫组化方法检测肾组织中OPN和MCP -1的表达及单核细胞抗原阳性 (ED1+ )细胞数。结果 H组大鼠血清总胆固醇和低密度脂蛋白增加 ,2 4h尿蛋白增加 ,而高密度脂蛋白和甘油三脂则无变化 ,内生肌酐清除率不受影响。光镜下见肾小球系膜细胞增多 ,系膜基质增加 ,肾小管上皮细胞浊肿 ,小管间质炎性细胞浸润。免疫组化显示与C组相比 ,H组大鼠肾OPN和MCP -1表达增加 ,主要位于皮质肾小管上皮细胞 ;ED1+ 细胞数增多 ,主要位于小管间质区域 ,OPN和MCP -1均与ED1+ 细胞数呈高度正相关。结论 高脂血症大鼠肾小管表达OPN和MCP -1增加 ,可能介导小管间质MΦ浸润 ,导致肾间质纤维化
Objective To investigate the expression and significance of osteopontin (OPN) and monocyte chemoattractant protein-1 (MCP-1) in hypercholesterolemic rats. Methods Twenty five Wistar rats were randomly divided into two groups: control group (group C, n = 10) and high fat diet group (group H, n = 15). Rats in group C were fed with normal rat feed, group H was fed with high-fat rat feed containing 4% cholesterol and 1% sodium cholate. Serum lipid components, 24 h urinary protein, creatinine clearance rate, HE The pathological changes of kidney were observed with PAS staining. The expression of OPN and MCP - 1 and the number of monocyte antigen positive (ED1 +) cells in kidney were detected by immunohistochemistry. Results In group H, serum total cholesterol and low density lipoprotein increased, while urinary protein increased at 24 h, while high density lipoprotein and triglyceride remained unchanged. Endogenous creatinine clearance was not affected. Light microscopic glomerular mesangial cells, increased mesangial matrix, renal tubular epithelial cell turbidity, tubulointerstitial inflammatory cell infiltration. Immunohistochemistry showed that compared with group C, the expression of OPN and MCP-1 in renal tissue of H group increased, mainly in cortical tubular epithelial cells; ED1 + cell number increased, mainly located in the tubulointerstitial region, OPN and MCP- ED1 + cell number was highly positive. Conclusions The expression of OPN and MCP-1 in the renal tubules of hyperlipidemic rats increases, which may mediate the MΦ infiltration in tubulointerstitial tissue, resulting in renal interstitial fibrosis