论文部分内容阅读
目的探讨原发性肝癌(HCC)组织中螺杆菌感染情况。方法选取经病理诊断的28例HCC患者的肝癌组织为实验组,22例非肝癌肝病患者肝组织和25例胃癌患者胃癌组织作对照组。采用聚合酶链反应(PCR)扩增螺杆菌属16S rRNA基因、幽门螺杆菌相关功能基因空泡毒素基因(vacA)和细胞毒素相关基因(cagA),16S rRNA的扩增产物经Southern杂交确认,并将PCR产物进行测序及同源性比较。结果28例HCC标本中有17例检出螺杆菌属16S rRNA基因,阳性率为60．7%；25例胃癌标本有18例检出螺杆菌16S rRNA,阳性率为72．0%；其他肝病组未扩增出16S rRNA基因。16S rRNA PCR产物经Southern杂交证实为幽门螺杆菌。序列测定表明,肝癌和胃癌组织中的螺杆菌16S rRNA序列与幽门螺杆菌序列有97．8%的同源性。肝癌组相关功能基因有3例cagA基因阳性,胃癌组有2例cagA基因阳性,均未扩增出vacA基因。提示,幽门螺杆菌菌株的基因型多为Ⅱ型,而少数为Ⅰ型。结论HCC患者肝组织中存在螺杆菌感染且感染率较高。螺杆菌感染与HCC可能存在相关性。
Objective To investigate the status of Helicobacter infection in primary liver cancer (HCC). Methods HCC tissues from 28 HCC patients diagnosed by pathology were selected as experimental group, 22 cases of non-HCC liver tissues and 25 cases of gastric cancer as control group. The 16S rRNA gene of Helicobacter, vacA and cagA of Helicobacter pylori-associated genes were amplified by polymerase chain reaction (PCR). The amplified product of 16S rRNA was confirmed by Southern blotting, The PCR products were sequenced and compared for homology. Results The positive rate of 16S rRNA gene of Helicobacter was detected in 17 out of 28 HCC specimens, and the positive rate was 60.7%. The positive rate of 16S rRNA of Helicobacter was detected in 18 of 25 gastric cancer specimens, and the positive rate was 72.0% The 16S rRNA gene was not amplified in the group. The 16S rRNA PCR product was confirmed as H. pylori by Southern blotting. Sequence analysis showed that the Helicobacter pylori 16S rRNA sequence in HCC and gastric cancer tissues had 97.8% homology with H. pylori sequence. There were 3 cases of cagA gene positive function in HCC group and 2 cases of cagA gene in gastric cancer group. No vacA gene was amplified. Tip, Helicobacter pylori strains genotype Ⅱ, and a few are type Ⅰ. Conclusion There is Helicobacter infection in the liver tissue of HCC patients and the infection rate is high. Helicobacter infection and HCC may be related.