将细胞包埋于凝胶基质中,用核磁共振(NMR)方法可以研究细胞内在的代谢规律、细胞系之间的生化差异以及药物、营养物、代谢物前体、激素和生长因子的作用。作者在一个10mm 的样品管中,用琼脂糖包埋细胞密度为2×10~8/1～2ml 的 MCF-7人乳癌细胞,进行灌流是为了提供营养物和消除细胞代谢过程中的废物。当药物作用时细胞仍能保持正常代谢的稳定性,可极其相似地模拟生理条件进行研究。磷脂生物合成酶是在膜与胞液界面上作用的,如对提取后的蛋白进行研究则不能代表真实的生理状态,而用无损伤细胞技术则是目前最为理想的。以γ-ATP 作为内标,~(31)P-NMR 研究乙醇胺对灌流 MCF-7细胞磷脂代谢的影响,可方便地提供磷酸乙醇胺 Embedding cells in a gel matrix allows the study of the inherent metabolic processes of cells, the biochemical differences between cell lines, and the effects of drugs, nutrients, metabolite precursors, hormones and growth factors using nuclear magnetic resonance (NMR) methods. In a 10-mm sample tube, MCF-7 human breast cancer cells were seeded with agarose at a cell density of 2 × 10 -8 / 1 ~ 2 ml for perfusion to provide nutrients and to eliminate wastes from cellular metabolism. When the drug effects the cells still maintain the normal metabolism of the stability can be very similar to simulate the physiological conditions of the study. Phospholipid biosynthesis enzyme in membrane and cytosolic interface role, such as the extracted protein can not represent the true physiological state of the study, but with non-invasive cell technology is the most ideal. The effect of ethanolamine on the phospholipid metabolism of perfusate MCF-7 cells was investigated by γ-ATP as internal standard and ~ (31) P-NMR. Phosphoethanolamine