论文部分内容阅读
应用快速酶联免疫法（ELISA）及Northern印迹杂交法研究了博莱霉素（BLM）同系物诱导癌基因表达的作用．通过检测P21和c－myc蛋白表达的改变和药物在RNA的转录水平上对癌基因表达的影响，证明BLM能够抑制c－myc基因的表达．这种抑制作用不仅发生在蛋白质的翻译水平，而且可能发生在RNA的转录水平上．BLMA6及A2对Ras基因亦有极显著的抑制，提示其亦为以p21蛋白为靶点的抗癌抗生素．A6、A2与A5之间的区别提示在同系物之间可能存在不同的抗癌机理
The effect of bleomycin (BLM) homologue on oncogene expression was studied by rapid enzyme-linked immunosorbent assay (ELISA) and Northern blot hybridization. By detecting changes in the expression of P21 and c-myc proteins and the effect of drugs on the expression of oncogenes in RNA transcription levels, it was demonstrated that BLM can inhibit the expression of c-myc genes. This inhibition not only occurs at the level of protein translation, but also at the RNA transcription level. BLMA6 and A2 also showed significant inhibition of Ras gene, suggesting that it is also an anticancer antibiotic targeting p21 protein. The difference between A6, A2 and A5 suggests that there may be different anti-cancer mechanisms between homologs