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目的调查Lewis血型系统抗体在备血患者中的检出率及分布情况。方法对抗体筛选阳性的标本做抗体鉴定。抗体筛选及交叉配血采用微柱凝胶抗球蛋白法及盐水介质试管法;抗体鉴定采用微柱凝胶抗球蛋白法;抗原鉴定采用试管法;并回顾分析本院Lewis血型系统抗体阳性患者的临床资料。结果 94例存在Lewis血型系统抗体患者中,抗-Lea89例,抗-Leb4例,抗-Le~a+Le~b1例,检出率分别是5.69×10~(-4)、0.26×10~(-4)、0.06×10~(-4);Lewis血型均为Le(a-b-);女性患者显著多于男性患者(P<0.01);妊娠期患者多见,共35例,占37.2%。17例输血患者,无1例发生溶血反应。结论 Lewis血型系统抗体意义值得探讨,盐水介质试管法和微柱凝胶抗球蛋白2种方法同时配血,可加强输血安全。
Objective To investigate the detection and distribution of Lewis blood group antibody in patients with blood preparation. Methods Antibodies were screened for positive antibodies for antibody identification. Antibody screening and cross matching with micro-column gel antiglobulin method and saline medium test tube method; antibody identification using microcolumn gel antiglobulin method; antigen identification using test tube method; and retrospective analysis of our hospital Lewis blood group antibody positive patients The clinical data. Results Among the 94 patients with Lewis blood group antibody, the detection rates of anti-Lea89, anti-Leb4 and anti-Lea + Le1b were 5.69 × 10 ~ (-4) and 0.26 × 10 ~ (-4) and 0.06 × 10 ~ (-4), respectively. Lewis blood type was Le (ab-). There were more female patients than male patients (P <0.01) . 17 cases of transfusion patients, no case of hemolytic reaction. Conclusion The significance of the Lewis blood group antibody is worth to be explored. Both the saline medium tube method and the microcolumn gel anti-globulin method can be used simultaneously to increase blood transfusion.