通过A、B、C、D 4株解磷解钾芽孢杆菌间互作效应研究,找出最佳菌株组合,为复合菌肥的研制提供技术支持。通过钼锑抗比色法、火焰分光光度计等方法分别测定了上述4株芽孢杆菌单独和混合培养菌株的溶磷、解钾能力与培养液pH和有机酸总量变化及其彼此之间的相关性。结果表明:在无机磷发酵培养液上清液中,菌株混合培养的各处理可溶性磷含量均明显高于单菌株处理,并且各处理的可溶性磷含量与pH成显著的负相关,而与有机酸总量成明显的正相关;在有机磷发酵培养液上清液中,各处理的可溶性磷含量差异不显著(P>0.05),而且可溶性磷含量与pH、有机酸总量之间不存在显著的相关性;在钾矿石发酵培养液上清液中,各处理均具有一定的溶钾能力,并且可溶性钾含量与其pH、有机酸总量之间、有机酸总量与pH之间均呈现一定的相关性,但各处理可溶性钾含量差异不显著(P>0.05)。各菌株混合培养,尤其是三菌和四菌株组合处理能显著提高可溶性磷含量,但并不完全表现为加成效应;综合各处理的特性,第13处理组组合菌株间协同效应较强,有望成为最佳菌株组合。 By A, B, C, D 4 strains of phosphate-solubilizing bacteria between the interaction between the study of Bacillus to identify the best strain combinations for the development of composite bacterial fertilizer to provide technical support. The solubilities and solubilities of the four strains of Bacillus isolated and mixed cultures were determined by molybdenum-antimony colorimetric method and flame spectrophotometer respectively. The changes of pH and total organic acids in culture broth and their relationship with each other Correlation. The results showed that in the inorganic phosphorus fermentation medium supernatant, the soluble phosphorus content in the mixed culture of the strains was significantly higher than that of the single strain, and the soluble phosphorus content of each treatment had a significant negative correlation with pH, There was a significant positive correlation between the content of soluble phosphorus and the total amount of soluble organic phosphorus in the supernatant of the organophosphorus fermentation broth. There was no significant difference between the contents of soluble phosphorus and total organic acids The correlation between soluble potassium content and pH, the total amount of organic acids, the total amount of organic acids and pH between the fermentation medium in potassium ore fermentation broth supernatant, However, there was no significant difference (P> 0.05) between the contents of soluble potassium in different treatments. The mixed culture of each strain, especially the combination of three bacteria and four strains can significantly increase the content of soluble phosphorus, but not entirely as an additive effect; comprehensive treatment of the characteristics of the combination of the first 13 groups of strong synergism between strains is expected Be the best strain combination.