高热是否可以直接诱发DNA单链断裂,文献意见尚不一致。Lunec等报告,高热处理的细胞来检测到DNA单链断裂。Dikomey等指出,高热或45℃以上处理的细胞,在37℃培养4～24小时便可出现DNA单链断裂。自从~6H-胸腺嘧啶核苷(~3H-TdR)用于实验细胞的DNA标记以来,认为DNA单链断裂可以由0H的内照射而诱发。为了探讨DNA单链断裂的原因,本文报告了EA(Ehrlich ascites)和HeLa S_3细胞在高热下处理后,X线照射与不照射时DNA单链断裂的发生情况。验证了是高热本身的直接或间接作用,还是标记DAN的~3H-TdR的~3H内照射诱发DNA单链断裂。同时也讨论了高热与辐射诱发的DNA单链断裂修复抑 High fever can directly induce DNA single strand breaks, the literature opinion is not the same. Lunec et al reported that heat-treated cells detect DNA single-strand breaks. Dikomey et al. Show that DNA single-strand breaks can occur in cells that are hyperthermic or above 45 ° C for 4 to 24 hours at 37 ° C. Since ~ 6H-thymidine (~ 3H-TdR) has been used for DNA labeling of experimental cells, it is believed that DNA single strand breaks can be induced by internal irradiation at 0H. In order to investigate the reason of DNA single-strand breaks, we report the occurrence of DNA single-strand breaks in EA (Ehrlich ascites) and HeLa S 3 cells treated with hyperthermia, with and without X-ray irradiation. The direct and indirect effects of hyperthermia per se were confirmed, and ~ 3H internal irradiation of ~ 3H-TdR labeled DAN induced DNA single-strand breaks. High temperature and radiation-induced DNA single-strand break repair was also discussed