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目的 检测 p1 6基因蛋白在胃癌组织、癌旁组织中的表达及其分布特点 ,分析其与胃癌临床病理学特征及预后的关系 .方法 采用 S- P免疫组织化学法对 53例胃癌组织及 35例癌旁组织进行 p1 6蛋白的定位观察 .结果 各病理类型胃癌组织、癌旁组织均有 p1 6基因蛋白表达 .阳性率分别为 62 .3% (33/53)和 88.6% (31 /35) ,阳性细胞的棕黄色颗粒主要位于细胞核 .胃癌 p1 6基因蛋白表达与性别、年龄、肿瘤部位在统计学上无差异 (P>0 .0 5) ;而与组织学类型、病理分级、淋巴结转移、临床病理分期在统计学上有差异 (P<0 .0 5) .p1 6蛋白阳性者 5年生存率 51 .0 %高于 p1 6蛋白阴性者 2 0 .0 % (P<0 .0 5) .结论 p1 6基因缺失和表达水平的改变与胃癌发生、发展密切相关 ,检测 p1 6基因蛋白表达可作为辅助临床判断胃癌的生物学行为及推测预后的指标
Objective To detect the expression and distribution of p16 gene in gastric cancer tissues and pericarcinomatous tissues and to analyze its relationship with the clinicopathological characteristics and prognosis of gastric cancer.Methods The expressions of p16 gene in 53 cases of gastric cancer and 35 cases of gastric cancer were detected by immunohistochemistry The expression of p16 protein was detected in adjacent tissues.Results The expression of p16 protein in all pathological types of gastric cancer tissues and adjacent tissues was 62.3% (33/53) and 88.6% (31/35) ), The brown granules of positive cells were mainly located in the nucleus.The expression of p16 protein in gastric cancer was not statistically different from that of sex, age and tumor (P> 0.05), but not with histological type, pathological grade, lymph node There was a statistically significant difference in the stage of metastasis and clinical pathology (P <0.05). The 5-year survival rate of 51 patients with positive p1 6 protein was 51.0% higher than that of patients with negative p1 6 protein 20.0% (P <0.05). 0 5) .Conclusion The deletion of p16 gene and the change of its expression level are closely related to the occurrence and development of gastric cancer. Detecting the expression of p16 gene protein may be used as an index to assist in the clinical judgment of biological behavior and prognosis of gastric cancer