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目的研究盐生植物多枝柽柳中抑制血管紧张素Ⅰ转换酶(ACE)活性的成分。方法采用硅胶柱色谱、葡聚糖凝胶柱色谱等方法对多枝柽柳活性部位进行分离,运用现代光谱技术鉴定化合物结构。通过HPLC法测定各萃取物及酚类化合物对血管紧张素Ⅰ转换酶的抑制活性。结果从多枝柽柳中分离得到3种酚类化合物、2种甾醇类化合物,分别为异阿魏酸(1),4-羟基-3,5-二甲醚苯甲醛(2),4-羟基-3,5-二甲醚苯甲酸(3),β-谷甾醇(4),胡萝卜苷(5)。依据萃取物的活性情况,对3种酚类化合物进行了抑制血管紧张素Ⅰ转换酶活性的测定,在质量浓度为25mg.mL-1时,化合物1的抑制活性最高,抑制率可达82%,化合物2和3亦具有一定的抑制活性。结论化合物2和3为首次从该植物分离得到。依据活性追踪方法,化合物1,2和3均具有一定的抑制血管紧张素Ⅰ转换酶活性。
Objective To study the inhibitory activities of angiotensin Ⅰ converting enzyme (ACE) in Halophytes of halophytes. Methods Silica gel column chromatography and Sephadex LH-20 column were used to separate the active fractions from Tamarix androssii, and the structures of the compounds were identified by modern spectroscopy. Inhibitory activity of each extract and phenolic compound on angiotensin I converting enzyme was determined by HPLC. Results Three kinds of phenolic compounds and two kinds of sterols were isolated from Tamarix ramosissima, and they were isoferulic acid (1), 4-hydroxy-3,5-dimethylbenzaldehyde (2), 4- -3,5-dimethyl ether benzoic acid (3), β-sitosterol (4), daucosterol (5). According to the activity of the extract, three kinds of phenolic compounds were tested for inhibiting the activity of angiotensin I converting enzyme. When the mass concentration was 25 mg.mL-1, the inhibitory rate of compound 1 was up to 82% , Compounds 2 and 3 also have some inhibitory activity. Conclusions Compounds 2 and 3 were isolated from this plant for the first time. Based on the activity-tracing method, compounds 1, 2 and 3 all have certain inhibition of angiotensin I converting enzyme activity.