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本文目的旨在采用原位肝单次灌注系统来研究肝外阻塞性黄疸对枯氏细胞清除能力的影响。灌注液是不含血红蛋白的克汉二氏碳酸氢钠缓冲液加0.5%葡萄糖、0.5%清蛋白及异硫氰酸盐萤光标记的胶乳微粒(直径0.75μm)。灌注液经门静脉灌入,流速恒定在25ml/分,共10分钟,温度37℃,从膈上下腔静脉中每隔一分钟收集流出液,用萤光测定仪定量测流出液中的萤光素,根据灌入液和流出液的萤光素差值计算枯否氏细胞的清除能力(KCCC),用原灌注液的百分率表示。实验取35只重约250～300g雄性鼠,按Lee方法剖腹结扎切断胆总管,制备肝外阻塞性黄疸模型。灌注分6组进行,每组12只大鼠,第一组对照,未做手术,第2组对照,做手术,但未结扎切断胆总管,
The purpose of this paper is to investigate the effect of extrahepatic obstructive jaundice on the ability of Schwann cells to scavenge using the single perfused liver system. The perfusate is hemoglobin-free KHH buffer plus 0.5% glucose, 0.5% albumin and isothiocyanate fluorescently labeled latex particles (0.75 [mu] m diameter). The perfusate was infused through the portal vein and the flow rate was constant at 25 ml / min for 10 minutes at a temperature of 37 ° C. The effluent was collected every minute from the superior vena cava and the superior vena cava. Fluorescein , The ability of Kushen cells to scavenge (KCCC) was calculated from the difference between the fluorescein concentration of the infusate and the effluent, expressed as a percentage of the original perfusate. Experimental take 35 weighing about 250 ~ 300g male rats, according to Lee method to cut the common bile duct ligation, preparation of extrahepatic obstructive jaundice model. Perfusion divided into 6 groups, each group of 12 rats, the first group of control, no surgery, the second group of control, surgery, but not ligation of the common bile duct,