论文部分内容阅读
为了研究外生菌根菌与外源Ca~(2+)是否可以协同对樟子松苗木生长的影响.本研究利用菌物学、生物化学和生态学等研究方法,通过对菌根化樟子松一年生苗木施加适当浓度的外源Ca~(2+),并对苗木各部分各项生长和生理指标进行检测.结果表明:(1)适当浓度的外源Ca~(2+)和外生菌根菌(ECMF)协同能够更好的促进樟子松苗木的生长.当Ca≤20mmol/L时,苗木高生长达到最大,为11.4cm和10.6cm,比对照高32.6%和23.3%.生物量与光合色素含量均高于不施Ca处理组.说明施用适当浓度的外源Ca可以放大菌根对苗木的促生作用.(2)适当浓度的外源Ca~(2+)和外生菌根菌协同能够更好的提升樟子松苗木的抗氧化能力.施Ca处理主要可以诱导苗木各部分CAT和POD活力上升,通过较高水平的抗氧化酶系,提高苗木质量.(3)适当浓度的外源Ca~(2+)和外生菌根菌协同能够更好的提高樟子松细胞耐渗透能力.本研究中,外源Ca~(2+),可以有效同外生菌根菌协同,增加苗木各部分的可溶性蛋白含量.通过主成分分析(PCA)表明,在各项渗透物质指标当中,可溶性蛋白的贡献率最大.表明外源Ca和外生菌根可以有效诱导植物提高体内可溶性蛋白含量,提高抗逆性.(4)菌根苗通过施用适当浓度的外源Ca~(2+)达到加大壮苗的目的是可行的.综合本研究的各项结果,不难看出,苗木的生长和生理指标的变化一致,生长迅速且健壮的苗木,其体内抗氧化酶系统和细胞中渗透物质的含量也相应的增高.在育苗初期,在苗圃或大田施用外源Ca~(2+)(或钙肥)与外生菌根菌协同以期达到更好的壮苗之效果,是可行的.
In order to study whether ectomycorrhizal fungi and exogenous Ca ~ (2+) can synergistically affect the growth of Pinus sylvestris var. Mongolica seedlings, the mycelial growth of Pinus sylvestris var.mongolica was studied in this study. Using mycological, biochemical and ecological methods, The results showed that: (1) Exogenous Ca2 + and Exogenous Ca2 + in appropriate concentration ECMF synergism can promote the growth of Pinus sylvestris var.mongolica seedlings.When Ca≤20mmol / L, the height growth of seedlings reached the maximum of 11.4cm and 10.6cm, 32.6% and 23.3% higher than that of the control. The biomass and photosynthetic pigment content were higher than those under Ca2 + -free treatment, which indicated that the exogenous Ca could amplify the promoting effect of mycorrhiza on the seedling. (2) The appropriate concentration of exogenous Ca2 + Mycorrhizal symbiosis can enhance the antioxidant capacity of Pinus sylvestris var.mongolica seedlings.Application of Ca treatment can mainly induce the increase of CAT and POD activity in all parts of seedlings and improve seedling quality through the higher level of antioxidant enzymes. ) The appropriate concentration of exogenous Ca 2+ and ectomycorrhizal fungi collaborated to better improve the osmotolerant capacity of Pinus sylvestris var. Mongolica.In this study, The exogenous Ca 2+ could effectively cooperate with the ectomycorrhizal fungi to increase the content of soluble protein in various parts of seedlings.The principal component analysis (PCA) showed that the contribution rate of soluble protein Which indicated that exogenous Ca and ectomycorrhiza could effectively induce plants to increase the content of soluble protein in the body and increase the resistance to stress. (4) Mycorrhizal seedlings increased the exogenous Ca 2+ by increasing the concentration of exogenous Ca 2+ The purpose is feasible.Considering the results of this study, it is not difficult to see that the growth of seedlings and physiological indicators of the same, rapid growth and robust seedlings, the body’s antioxidant enzyme system and the content of cells in the corresponding permeate In the early seedling stage, nursery or field application of exogenous Ca ~ (2 +) (or calcium fertilizer) and ectomycorrhizal synergism in order to achieve better seedling effect is feasible.