目的:本研究以HepG2.2.15(2215)细胞为细胞模型,探讨芒果苷对2215细胞内β-arrestins信号通路的影响。方法:采用免疫印迹法(Western blotting)分别检测芒果苷对2215细胞中β-arrestins总蛋白含量、β-arrestins磷酸化(p-β-arrestins)水平以及EGF干预后芒果苷对2215细胞中p-β-arrestins水平的影响。结果:在无EGF干预时,芒果苷各剂量组对2215细胞中β-arrestins总蛋白含量及p-β-arrestins水平无影响;在EGF干预后,芒果苷100μg/ml能明显抑制2215细胞中p-β-arrestins水平;芒果苷50μg/ml、25μg/ml对β-arrestins磷酸化程度无影响。结论:抑制2215细胞中p-β-arrestins水平是芒果苷抗HBV和免疫调节作用的可能机制之一。 Objective: In this study, HepG2.2.15 (2215) cells were used as cell models to investigate the effect of mangiferin on the β-arrestins signaling pathway in 2215 cells. Methods: The total protein content of β-arrestins, the level of β-arrestins phosphorylation (p-β-arrestins) of mangiferin in 2215 cells were detected by Western blotting, and the effect of mangiferin on the expression of p- β-arrestins levels. Results: In the absence of EGF, the dosage of mangiferin had no effect on the total protein content of β-arrestins and the level of p-β-arrestins in 2215 cells. After EGF intervention, 100μg / ml of mangiferin significantly inhibited the p- -β-arrestins. Mangiferin 50μg / ml, 25μg / ml had no effect on the degree of β-arrestins phosphorylation. Conclusion: Inhibition of p-β-arrestins in 2215 cells is one of the possible mechanisms of anti-HBV and immunomodulatory effects of mangiferin.