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近几年脑内微量透析技术在国外已广泛应用于研究脑细胞间隙液中神经递质的变化,国内尚未见这方面的报道。本文用此技术,观察大鼠在不同程度缺血情况下,海马细胞外液中兴奋性氨基酸(EAA)(谷氨酸Glu,天门冬氨酸ASP)的变化。用0.3mm直径的透析管横穿大鼠双侧海马,随后用人工脑脊液以1μl/min的速度灌注。脑缺血模型用电解式氢清除法测定脑血流的变化。短暂性不完全性脑缺血,Glu和Asp分别升高到8.9±4.8,1.09±0.4μmol。短暂性完全性脑缺血,Glu、Asp分别升高到18.4±13.?,3.9±3.1μmol。这些变化随血流恢复而复原。持续性完全性脑缺血Glu、Asp分别升高到61.7±16.8,13.4±9.0μmol。相关系数r=0.646,证实脑缺血程度与EAA释放呈正相关。
In recent years, intracerebral microdialysis technology has been widely used in foreign countries to study the changes of neurotransmitters in brain cell interstitial fluid, and no report on this aspect has been reported in China. In this paper, we observed the changes of excitatory amino acids (EAA) (glutamic acid Glu, aspartic acid ASP) in hippocampus extracellular fluid of rats under different degrees of ischemia. The bilateral hippocampus of rats was traversed with a 0.3 mm diameter dialysis tube and then perfused with artificial cerebrospinal fluid at a rate of 1 μl / min. Changes of cerebral blood flow measured by electrolytic hydrogen scavenging in cerebral ischemia model. Transient incomplete cerebral ischemia, Glu and Asp were increased to 8.9 ± 4.8,1.09 ± 0.4μmol. Transient global cerebral ischemia, Glu, Asp were increased to 18.4 ± 13.?, 3.9 ± 3.1μmol. These changes recover with blood flow recovery. Persistent complete cerebral ischemia Glu, Asp were increased to 61.7 ± 16.8,13.4 ± 9.0μmol. Correlation coefficient r = 0.646, confirmed that the degree of cerebral ischemia and EAA release was positively correlated.