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应用单个脾结节移植和性染色体追踪方法证明正常F_1小鼠骨髓脾结节生成细胞(CFCs)的造血重建能力是很不均一的,连续注射羟基脲(HU)可使具有造血重建能力的脾结节生成细胞的比例显著提高。由此提出,小鼠造血组织中同时存在着具有多向性造血干细胞特性的脾结节生成细胞(Ps-CFUs)和具有多向性造血祖细胞特性的脾结节生成细胞(Pg-CFUs)。注射HU可使Pg-CFUs被杀灭,Ps-CFUs 相对比例增加;或者由于大量 CFU-S被杀灭,脾结节生成细胞的前体细胞(Pre-CFUs)加速向 Ps-CFUs分化。因此血细胞的生成过程中可能经历了以下细胞分化和成熟的阶段 Pre-CFUs→Ps-CFUs→Pg-GFUs→各系祖细胞→各系血细胞。
Single spleen nodule transplantation and sex-chromosome tracing methods were used to demonstrate that the ability of hematopoietic reconstitution of bone marrow splenic nodule-forming cells (CFCs) in normal F_1 mice is very heterogeneous. Continuous injection of hydroxyurea (HU) can make spleen with hematopoietic reconstitution ability The proportion of nodule-producing cells was significantly increased. Therefore, it is suggested that spleen nodule-producing cells (Ps-CFUs) with multipotential hematopoietic stem cells and spleen nodule-producing cells (Pg-CFUs) with multipotential hematopoietic progenitor cells exist in the hematopoietic tissues of mice, . Pg-CFUs can be killed by injection of HU, the relative proportion of Ps-CFUs increases, or pre-CFUs of splenic nodule-producing cells accelerate to differentiate into Ps-CFUs due to the killing of large numbers of CFU-S. Therefore, the generation of blood cells may have undergone the following stages of cell differentiation and maturation: Pre-CFUs → Ps-CFUs → Pg-GFUs → various lineage progenitor cells → all lineal blood cells.