采用纸片法和琼脂平板稀释法测定了 196株临床分离菌对司帕沙星的敏感性 ,并同洛美沙星、氧氟沙星、环丙沙星和依诺沙星进行比较。纸片法结果显示 ,细菌对司帕沙星的敏感率最高 ,为 97.45 % ;其次是环丙沙星 (93.36 % ) ,氧氟沙星 (92 .85 % ) ,洛美沙星 (91.32 % ) ;对依诺沙星的敏感率最低 (84.6 9% )。革兰氏阳性菌对司帕沙星的敏感率略高于革兰氏阴性菌 ,分别为 10 0 %和 96 .6 8% (P>0 .0 5 )。金葡球菌、大肠埃希氏菌、铜绿假单胞菌和不动杆菌对司帕沙星的敏感率分别为 10 0 %、94.12 %、95 .0 0 %和 88.89%。MIC测定结果表明 ,司帕沙星对所有细菌的 MIC均低于其它 4种抗菌药物。抑制 5 0 %和 90 %的金葡球菌所需的司帕沙星浓度为 0 .12 5和 2 mg/ L,是其它药物 1/ 4～ 1/ 16。对于肠杆菌科的细菌 ,司帕沙星的 MIC5 0 和 MIC90 在 0 .5和 4mg/ L 之间 ,是其它 4种药物 1/ 4～ 1/ 8倍。对于铜绿假单胞菌 ,司帕沙星的 MIC5 0 和 MIC90 与环丙沙星和依诺沙星相同 ,低于洛美沙星和氧氟沙星。对不动杆菌的体外抗菌活性优于其它 4种药物 4～ 8倍。所有 5种氟喹诺酮药物对沙门氏菌属和其它肠杆菌均显示非常好的体外抗菌活性。 MIC90 在 <0 .0 6～ 0 .5 mg/ L之间。以上说明 ,司帕沙星较洛美沙星、氧氟沙? The sensitivity of 196 clinical isolates to sparfloxacin was determined by the disk method and agar plate dilution method, and compared with lomefloxacin, ofloxacin, ciprofloxacin and enoxacin. The results of the disk method showed that the sensitivity of bacteria to sparfloxacin was 97.45%, followed by ciprofloxacin (93.36%), ofloxacin (92.85%) and lomefloxacin (91.32%), The lowest susceptibility to enoxacin (84.6 9%). The sensitivity of Gram-positive bacteria to sparfloxacin was slightly higher than that of gram-negative bacteria, which was 100% and 96.68%, respectively (P> 0.05). Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter were sensitive to sparfloxacin were 100%, 94.12%, 95.0% and 88.89% respectively. MIC measurement results showed that sparfloxacin MIC for all bacteria were lower than the other four antibacterial drugs. The sparfloxacin concentrations required to inhibit 50% and 90% of Staphylococcus aureus were 0.125 and 2 mg / L, among other drugs, 1/4 to 1/16. For Enterobacteriaceae, sparfloxacin has a MIC50 and MIC90 between 0.5 and 4 mg / L, which is 1/4 to 1/8 times that of the other four drugs. For Pseudomonas aeruginosa, sparfloxacin MIC50 and MIC90 were the same as ciprofloxacin and enoxacin, lower than lomefloxacin and ofloxacin. The antibacterial activity of Acinetobacter in vitro is 4 ~ 8 times better than the other 4 drugs. All five fluoroquinolones showed very good in vitro antibacterial activity against Salmonella and other Enterobacteriaceae. MIC90 is between <0.06 and 0.5 mg / L. Sparfloxacin than lomefloxacin, ofloxacin?