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目的:获得SARS冠状病毒蛋白与caveolin-1蛋白相互作用的信息,识别病毒蛋白中可能的caveolin-1结合位点,为SARS冠状病毒蛋白的功能研究以及设计抗SARS病毒的药物 和疫苗提供线索。方法:基于3个caveolin结合基序,采用氨基酸基序搜索方法预测SARS冠状病毒蛋白中可能与caveolin-1相互作用的区域,并用分子模拟和分子对接的方法进一步证实它们之间的相互作用。结果:通过生物信息学分析,在SARS冠状病毒蛋白中发现了36个caveolin结合基序,经过分子模拟和分子对接,获得了SARS冠状病毒蛋白与caveolin-1相互作用的8个结合位点,这些caveolin-1结合位点分别位于replicase 1AB、S蛋白、ofr3蛋白和M蛋白。结论:caveolin-1可能是SARS冠状病毒蛋白结合的一个靶点,它们之间的相互作用可能与SARS冠状病毒的感染、复制、装配和释放有关。
OBJECTIVE: To obtain information on the interaction between SARS coronavirus protein and caveolin-1 protein, to identify possible caveolin-1 binding sites in viral proteins, to provide clues for the functional study of SARS coronavirus protein and to design drugs and vaccines against SARS virus. Methods: Based on the three caveolin binding motifs, the amino acid motif search method was used to predict the possible regions of SARS coronavirus protein interacting with caveolin-1, and the molecular interactions and molecular docking were used to confirm their interaction. RESULTS: Thirty-six caveolin binding motifs were found in the SARS coronavirus protein by bioinformatics analysis. Eight binding sites of the SARS coronavirus protein interacting with caveolin-1 were obtained by molecular modeling and molecular docking The caveolin-1 binding site is located on the replicase 1AB, S protein, ofr3 protein and M protein, respectively. CONCLUSION: Caveolin-1 may be a target of SARS-CoV protein binding and the interaction between them may be related to infection, replication, assembly and release of SARS-CoV.