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目的:探讨DC-SIGNR在人胎盘绒毛组织中的定位及在体外培养滋养层细胞上的表达。方法:建立稳定的滋养层细胞原代培养体系,采用免疫组化单染及荧光双染的方法检测不同孕期正常胎盘绒毛组织及体外培养滋养层细胞上DC-SIGNR的表达。结果:DC-SIGNR主要表达于胎盘滋养层细胞、Hofbauer细胞及胎盘微血管内皮细胞的胞质及包膜,在原代培养的人绒毛膜滋养层细胞中的DC-SIGNR的表达与在体组织表达一致。结论:DC-SIGNR表达于不同孕期的胎盘组织及体外分离培养滋养层细胞,为研究滋养层细胞上该受体在宫内感染中的作用提供体外实验的细胞学基础。
Objective: To investigate the localization of DC-SIGNR in human placenta villi and its expression on trophoblast cells in vitro. Methods: A stable primary culture system of trophoblast cells was established. The expression of DC-SIGNR on normal placental villi and trophoblast cells in different stages of pregnancy was detected by immunohistochemical staining and double staining. Results: DC-SIGNR was mainly expressed in the cytoplasm and envelope of placental trophoblastic cells, Hofbauer cells and placental microvascular endothelial cells. The expression of DC-SIGNR in primary cultured human chorionic trophoblast cells was consistent with the expression in tissue . CONCLUSION: DC-SIGNR is expressed in the placenta of different pregnancy stages and isolated from trophoblast cells in vitro. It provides a cytological basis for studying the role of this receptor in trophoblast cells in intrauterine infection.