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从培养不同时间后的柱状嗜纤维菌中提取菌体脂多糖 (LPS) ,测定了其中蛋白质含量及成分的变化 ,并分别作为免疫原接种翘嘴鳜对其免疫原性进行了比较。结果表明 ,培养时间对菌体LPS中蛋白质含量没有明显影响 ,对其蛋白质成分则有一定影响 ,而菌体LPS中蛋白质成分与其免疫原性有关 ,从培养 36h的菌体中提取的菌体LPS的免疫原性最强
The lipopolysaccharide (LPS) was extracted from the columnar strain of fibroblasts cultured at different times, and the content and composition of the lipopolysaccharide were determined. The immunogenicity of the lipopolysaccharide was compared with that of the immunogen. The results showed that the culture time had no significant effect on the protein content of LPS, but had some influence on its protein composition. The protein components in LPS were related to its immunogenicity. The LPS The most immunogenicity